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For the thio-amide substrates, the Cd-enzyme was the very best enzyme and the Zn-enzyme was poorer. The catalytic constants for that Zn- and Cd-substituted varieties of NagA assist the summary which the carbonyl team of your substrate is polarized by a immediate conversation with The only divalent cation bound to the Mβ-situation while in the Lively internet site.
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m vs. pH-profile for Zn-NagA is shown in Figure 2B. With this profile, catalytic activity is shed at higher and reduced values of pH and the info link naga26 were being fit to equation 4 the place two teams, in both the substrate or free of charge enzyme, with similar p
-acetyl-d-glucosamine-6-phosphate as illustrated in Scheme 1. The deacetylation of this compound provides a source of carbon and nitrogen by making ready this substrate for entry to the glycolytic pathway. This reaction is actually a key move from the catabolism of N
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